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DEG Assay

DEG Assay is a new analytical method which can detect small amounts of electrophoretically separated proteins with high sensitivity. By using sainome_®, you can perform DEG Assay efficiently and with high reproducibility.

Feature1 : Activity-based discovery of novel enzymes

DEG assay enables the selective detection of the protein with specified activities on electrophoresis gel.
Protein less than 0.1ng can be detected.

*data from J. Am. Chem. Soc. 2013, 135, 6002-6005.

Feature 2 : Isozyme-selective study of enaymes

More than 100 fluorescent probes for certain enzymes are commercially availeble.
*data from J. Am. Chem. Soc. 2013, 135, 6002-6005.

Examples of fluorescent probes

	Enzyme	Probe	Detection Limit
Glycosidases	β-Galactosidase	TGβ-Gal	0.03ng
	β-Glucosidase	TGβ-Glc	1ng
Esterases	Alkaline Phosphatase	Umb Phos	0.3ng
	Porcine Liver esterase (PLE)	Fluorescin Dibutyrase (FDB)	10ng
Peptidases	Elastase	Meo-Suc-Ala-Ala-Pro-lle-AMC	3ng
	Leucine Aminopeptidase (LAP)	Leu-AMC	0.3ng

Feature 3 : Applicable to valiable detection needs of enzymatic activities

Applicable to search of enzymatic activity which linked to certain phenotype.
(example：Fluorescentprobe for APEH, protein characterized as N-formyl methionine peptide metabolizing enzyme.

*data from J. Am. Chem. Soc. 2013, 135, 6002-6005.

Applications : Applicable to biological and clinical experiments of valious enzymes

- Discovery of 2 formylated peptides metabolizing enzymes.
- Comprehensive analysis of oxidized protein-metabolizing enzymes.(see figure below)
- Detection of alkaline phosphatase activities in serum.

References

- Kentaro Yoshioka, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, and Yasuteru Urano, Chem. Commun., 52, 4377-4380 (2016)
- Kentaro Yoshioka, Toru Komatsu, Akihiro Nakada, Jun Onagi, Yugo Kuriki, Mitsuyasu Kawaguchi, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, and Yasuteru Urano, J. Am. Chem. Soc., 2015, 137, 12187-12190
- Toru Komatsu and Tetsuo Nagano, MEDCHEM NEWS No. 3 AUGUST 2013 .33
- Toru Komatsu, Kenjiro Hanaoka, Alexander Adibekian, Kentaro Yoshioka, Takuya Terai, Tasuku Ueno, Mitsuyasu Kawaguchi, Benjamin F. Cravatt, and Tetsuo Nagano, J. Am. Chem. Soc. 2013, 135, 6002-6005